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White ProxiPlate 384-well microplate

白色ProxiPlate 384孔微孔板

公司名称: PerkinElmer
产品编号: 6008280
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pERK Detection Assays Using the Surefire AlphaScreen® Kit (TGR Biosciences and PerkinElmer)
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Date:
2013-07-05
[Abstract]  Extracellular signal-regulated kinase 1 and 2 (ERK1/2) are serine/threonine protein kinases that are phosphorylated on Thr202/Tyr204 (ERK1) and Thr185/Tyr187 (ERK2). Phosphorylation of ERK1/2 (pERK1/2) arises from multiple stimuli, resulting in physiological responses that include cell growth, proliferation and differentiation. This protocol has been optimized for the detection of ligand-mediated pERK1/2 in adherent immortal cell lines expressing G protein-coupled receptors (GPCRs). [摘要]  细胞外信号调节激酶1和2(ERK1/2)是在Thr202/Tyr204(ERK1)和Thr185/Tyr187(ERK2)上被磷酸化的丝氨酸/苏氨酸蛋白激酶。 ERK1/2(pERK1/2)的磷酸化来自多个刺激,导致包括细胞生长,增殖和分化的生理反应。 该协议已经优化用于检测表达G蛋白偶联受体(GPCR)的贴壁永生细胞系中配体介导的pERK1/2。

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