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Author:
Date:
2013-06-05
[Abstract] Transposable elements (TEs) are repetitive sequences, capable of inducing genetic mutations through their transpositional activity, or by non-homologous or illegitimate recombination. Because of their similarity and often high copy numbers, examining the effects of mutations caused by TEs in different samples (tissues, individuals, species, etc.) can be difficult. Thus, high throughput methods have been developed for genotyping TEs in un-sequenced genomes. A common method is termed Transposon Display (or transposon SSAP), which utilizes restriction enzymes and PCR amplification to produce ...
[摘要] 转座元件（TE）是重复序列，能够通过其转座活性或通过非同源或非同源重组诱导遗传突变。 由于它们的相似性和通常高的拷贝数，检查由不同样品（组织，个体，物种等）中TE引起的突变的影响可能是困难的。 因此，已经开发了用于在未测序的基因组中对TE进行基因分型的高通量方法。 常见的方法称为转座子展示（或转座子SSAP），其利用限制酶和PCR扩增来产生包括基因组和TE DNA的嵌合DNA分子。 第二代测序技术（例如454焦磷酸测序）的出现已显着改善了该测定的分辨率，允许同时测序所有PCR产物，代表特定基因组中的所有扩增的TE位点。
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