| In vitro Floral Induction of Cuscuta reflexa
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Author:
Date:
2017-01-20
[Abstract] Floral initiation and development in the angiosperms is the essential step on which the yield of the plant depends. Sometimes external climate or any abiotic stress hinders the floral initiation and ultimately affect the plant yield. Hence, in vitro floral induction and development can help to overcome the external climatic factor. Furthermore, the protocol for in vitro floral induction in the parasitic angiosperm like Cuscuta reflexa has not been reported yet. We have standardized the protocol for floral induction in the parasitic plant Cuscuta reflexa. In ...
[摘要] 被子植物的花卉引发和发育是植物产量依赖的关键步骤。有时，外部气候或任何非生物胁迫阻碍花卉开始，最终影响植物产量。因此，体外花卉诱导和发育可以帮助克服外部气候因素。此外，尚未报道如在睾丸反射中的寄生被子植物体外花诱导的方案。我们已经规定了寄生植物茧丝反射中花诱导的方案。在本研究中，确定补充有2mg L NAA（萘乙酸，植物生长调节剂）和2mg L -1的MMS（改良的Murashige Skoog）培养基> 2,4-D（2,4-二氯苯氧基丙酸，植物生长调节剂）在体外拍摄后支持花诱导。此外，我们发现补充有2mg L-sup-2,4-D的MMS培养基从淋巴结外植体直接快速诱导花蕾，而没有任何芽伸长。该协议将有助于研究人员在其他被子植物植物中诱导花along与镰刀菌反射。背景 睾丸反射是寄生在大量被子植物上寄生的被子植物（Kuijt，1969）。在宿主植物中造成严重损害和产量损失的能力使得该物种对科学研究非常重要（Nun and Mayer，1999）。除了少数具有功能性叶绿体（Hibberd等人，1998）之外，大多数Cuscuta 物种是非叶绿体。从较大的胚胎开发幼苗。已经报道了白色介质中的反射（Maheshweri和Baldev，1961）。花卉诱导。反射炎在短时间内以及黑暗条件已经在体外中看到（Baldev，1962）。 ...
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| Hydroponic Culture of ‘Micro-Tom’ Tomato
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Author:
Date:
2015-10-05
[Abstract] We use ‘Micro-Tom’ to study tomato fruit ripening and development mechanisms. ‘Micro-Tom’ is suitable for cultivation and experiments due to its small size of 10 to 20 cm in height and short life cycle of 3 months. There is also an abundance of publically available information on ‘Micro-Tom’ including EST, full-length cDNA clones and transcriptome data. ‘Micro-Tom’ plants are grown in hydroponic culture under fluorescents using Arabidopsis cultural shelves in greenhouses or plant rooms to get data with reproducibility for transcriptome and proteome analyses.
[摘要] 我们使用"微汤姆"来研究番茄果实成熟和发育机制。 "微汤姆"适合于培养和实验，由于其小尺寸10至20厘米的高度和短的生命周期3个月。 还有大量关于"Micro-Tom"的公开信息，包括EST，全长cDNA克隆和转录组数据。 "Micro-Tom"植物在水培培养基中在荧光下使用在温室或植物室中的拟南芥文化架来生长，以获得具有转录组和蛋白质组分析的可重复性的数据。
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| Measurement of Potassium Content in Arabidopsis
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Author:
Date:
2013-12-05
[Abstract] Potassium is an essential element in plant growth and has an important role in regulating cell water potential and turgor in osmotic regulation. Potassium content in plants is high compared to trace elements, however, it is difficult to measure a relatively small change of potassium content in the large total. Here, we describe a procedure for measuring potassium in Arabidopsis that is easy to handle in preparative scale and avoids contamination.
[摘要] 钾是植物生长中的必需元素，并且在调节细胞水势和渗透调节中的膨胀中具有重要作用。 与微量元素相比，植物中的钾含量高，然而，难以在大的总量中测量钾含量的相对小的变化。 在这里，我们描述了在拟南芥中测量钾的程序，其易于在制备规模中处理并避免污染。
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