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Author:
Date:
2013-08-05
[Abstract] Extravillous trophoblast (EVT) migration and invasion through the decidualized endometrium is essential to successful placentation. SGHPL-4 cells, an EVT cell line derived from first trimester placenta, is a widely used model of cytotrophoblast differentiation into an invasive phenotype. Here we describe a quantitative cell migration assay that can be modified to also measure cell invasion. SGHPL-4 cells were seeded into BD Fluoroblok cell culture inserts constructed with an 8 μm porous membrane and allowed to migrate towards epidermal growth factor, a known chemoattractant for EVTs. To ...
[摘要] Extravillous滋养层（EVT）迁移和通过蜕膜化子宫内膜的侵入是成功胎盘成形的关键。 SGHPL-4细胞，来自前三个月胎盘的EVT细胞系，是广泛使用的细胞滋养层分化为侵袭性表型的模型。在这里我们描述了一种定量细胞迁移实验，可以修改也测量细胞入侵。将SGHPL-4细胞接种到用8μm多孔膜构建的BD Fluoroblok细胞培养插入物中，并允许其向表皮生长因子（一种已知的EVTs化学吸引剂）迁移。为了评估EVT侵入，首先用Matrigel（基底膜基质）包被Fluoroblok插入物。用钙黄绿素AM标记SGHPL-4细胞，并通过底部读数荧光读板器定量已经侵入和/或迁移穿过膜的细胞。 Fluoroblok插入物优于其他迁移/侵袭测定的优点是它们允许迁移细胞的非破坏性检测。
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