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Author:
Date:
2013-08-20
[Abstract] Yeast sphingolipids can be metabolically labeled with exogenously added radioactive precursors. Here we describe a method to label ceramides and complex sphingolipids of Saccharomyces cerevisiae with a radioactive ceramide precursor, D-erythro-[4, 5-3H]dihydrosphingosine. This protocol is used to study the biosynthesis, transport and metabolism of sphingolipids in yeast.
[摘要] 酵母鞘脂可以用外源添加的放射性前体代谢标记。 在这里我们描述了用放射性神经酰胺前体D-赤式 - [4,5] 3 H]二氢鞘氨醇标记神经酰胺和酿酒酵母的复杂神经鞘脂的方法。 该方案用于研究酵母中鞘脂的生物合成，转运和代谢。
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