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96 well plate reader

96孔板读数器

公司名称: Beckman Coulter
产品编号: Paradigm1113
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Pyruvate:ferredoxin Oxidoreductase (PFR1) Activity Assays Using Methyl Viologen as Artificial Electron Acceptor
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2013-09-05
[Abstract]  Here we describe the activity measurements of heterologous expressed pyruvate:ferredoxin oxidoreductase (Noth et al., 2013) (Noth et al.,2013) from Chlamydomonas reinhardtii. This enzyme catalyzes the reversible reaction (I) from pyruvate to acetyl CoA and CO2 generating low potential electrons which are in vivo transferred to ferredoxin.



In this assay we use methyl viologen as artificial electron acceptor which ...
[摘要]  这里我们描述了来自衣藻衣藻的异源表达丙酮酸:铁氧还蛋白氧化还原酶(Noth等,2013)(Noth等,2013)的活性测定。 该酶催化从丙酮酸向乙酰CoA的可逆反应(I)和产生低电位电子的CO 2,其将体内转移至铁氧还蛋白。

在该测定中,我们使用甲基紫精作为人造电子受体,其在其还原状态下变成深紫色(ε604= 13.6mM-1cm-1)(Mayhew,1978)(图1)。

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