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Author:
Date:
2013-10-20
[Abstract] Trehalose is a nonreducing disaccharide. It is a common sugar in bacteria, fungi and yeast, where it functions as a carbon source and stress protectant. In contrast, plants, although encoding large trehalose biosynthesis gene families, contain only small amounts of trehalose. The intermediate compound of trehalose, trehalose-6-phosphate (T6P), is a signaling molecule in plants, regulating metabolism, growth, and development. Most plants contain only a single trehalase, the enzyme that specifically hydrolyzes trehalose into two glucose molecules. High trehalase activity has been suggested to ...
[摘要] 海藻糖是非还原二糖。它是细菌，真菌和酵母中的常见糖，其在其中用作碳源和应激保护剂。相反，植物虽然编码大的海藻糖生物合成基因家族，但仅含有少量的海藻糖。海藻糖，海藻糖-6-磷酸（T6P）的中间体化合物是植物中的信号分子，调节代谢，生长和发育。大多数植物仅含有单一海藻糖酶，该酶特异性地将海藻糖水解成两个葡萄糖分子。已经提出高海藻糖酶活性是植物接种菌根真菌，根瘤菌和植物病原体禾本科植物的防御机制的一部分。最近，在拟南芥中显示高海藻糖酶活性与干旱胁迫耐受性的增加相关，并且海藻糖酶在气孔调节中实现重要作用。在这里，我们描述了测定拟南芥组织中的海藻糖酶活性的方案，优化用于96孔板。透析的蛋白质提取物将与海藻糖一起温育，随后使用葡萄糖氧化酶 - 过氧化物酶定量释放的葡萄糖。
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