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Author:
Date:
2013-11-20
[Abstract] Medicago truncatula serves as a model plant for legume genetics and genomics. We used RNA-Seq to characterize the transcriptome during the early organogenesis of the nodule and during its functioning. We generated approximately 135.5 million high-quality 36-bp reads, which were then aligned with the M. truncatula genome sequence (Mt3.0 version) and with sequences from a custom splice-junction database, for the detection of transcribed regions and splicing sites. Mapping and analysis of the reads conducted to the detection of 37,333 expressed transcription units (TUs), 1,670 ...
[摘要] Medic属truncatula 是豆科植物遗传学和基因组学的模型植物。我们使用RNA Seq表征转录组在结节的早期器官发生期间和其功能期间。我们产生了大约135.5百万高质量的36-bp读数，然后与M比对。 truncatula基因组序列（Mt3.0版本）和来自定制剪接结数据库的序列，用于检测转录区和剪接位点。对进行到37,333个表达的转录单位（TU）的检测的读取的映射和分析，1,670以前从未被描述过并且被功能性注释。我们确定7,595新转录区，大部分对应于5'和3'UTR扩展和新的外显子与5,264以前注释的基因相关联。我们还通过进行Cufflinks分析以确定各种可变剪接形式的频率来评估结瘤转录组中的复杂性。因此，我们确定23,164不同转录物来自6,587基因。最后，我们进行了差异表达分析，其提供了在结瘤期间转录重编程的全面视图。
所有Illumina序列数据已被保存在NCBI短读档案中，Sanger测序PCR产物已被保藏GenBank（SRA048731）。长于200bp的装配的重叠群已经保藏在TSA（JR366937-JR375780）。覆盖率数据可在 http://ddlab.sci.univr.it/cgi -bin/gbrowse/medicago/ ...
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