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Isolation and Enrichment of Major Primary Neuroglial Cells from Neonatal Mouse Brain

Santosh Kumar Samal, Madhav Sharma and Jayasri Das Sarma

Published online: 2024-01-20

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Midbrain Neuron-glia Mixed Cultures

Author:

Huiming Gao,

Date:

2012-04-05

[Abstract] Mixed neuron-glia cultures provide a unique tool to study cellular contribution and molecular pathways in various neurological disorders. They are also invaluable for exploring neuron-glia interaction under physiological and pathological conditions. The relatively long-lasting midbrain neuron-glia mixed cultures generated following this protocol have been widely used to study the pathogenesis of Parkinson’s disease, the most common neurodegenerative movement disorder. [摘要] 混合神经元-神经胶质细胞培养提供了一个独特的工具来研究细胞的构成和各种神经系统疾病的分子途径。他们在探索生理和病理条件下神经元-胶质细胞相互作用方面大有用处。按照操作指南上相对持久的中脑的神经元—神经胶质细胞混合培养已被广泛用于研究帕金森氏症——最常见的神经性运动障碍疾病的发病机制。这份指南经过了Dr. Hong实验室不同研究人员尤其是Dr. Bin Liu多年的改进。

Generation of Neuron-enriched Cultures (Method 1)

Author:

Huiming Gao,

Date:

2011-11-05

[Abstract] This protocol will guide you through the process for generating midbrain neuronal cultures from late embryo mouse brain. These cultures serve as a useful tool to study molecular pathways during neuronal death in various neurological disorders. This method uses cytosine β-D-arabinofuranoside in the cultures to suppress the proliferation of glial cells. Seven days after the seeding, the neuron-enriched cultures prepared following this protocol will contain less than 10% glia cultures. The concentrations of β-D-arabinofuranoside should be adjusted according to your culture condition. This ... [摘要] 本方法将引导您完成从后期胚胎小鼠大脑产生中脑神经元培养的整个过程。这种培养是来研究在神经元死亡中各种神经系统疾病的分子通路的有用工具。这种方法使用胞嘧啶β-D-arabinofuranoside（阿糖胞嘧啶）到培养基中，抑制胶质细胞增生。播种后第7天，在本方法后的神经元富集的准备将含有不少于10％的神经胶质细胞培养基。β-D-arabinofuranoside的浓度应根据培养基条件调整。此实验方法经Dr. Hong实验室不同研究人员多年的发展和完善，尤其是Dr. Bin Liu.

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