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Author:
Date:
2012-04-20
[Abstract] PI3-kinases regulate a wide range of cellular responses through the production of phosphatidylinositol 3, 4, 5-trisphosphate [PI (3, 4, 5) P (3)] in cellular membranes. This protocol provides a highly efficient and easy-to-handle method for sensitive detection and quantification of PI 3-kinase activity.
[摘要] 肌醇磷脂3通过在细胞膜上产生磷脂酰肌醇3,4,5-三磷酸调控一个大范围的细胞应答。这个步骤提供一个高效、简单操作的方法为敏感检测和肌醇磷脂3-激酶活性的定量。
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