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Author:
Date:
2013-10-05
[Abstract] LDH (Lactate dehydrogenase) enzyme catalyzes the reversible conversion of pyruvate to lactate using NAD+ as a cofactor. Although the physiological significance of lactate accumulation in tumor cells, a dead-end product in cellular metabolism, is currently a topic of debate, it has long been known that many tumor cells express a high level of LDH-A (Koukourakis et al., 2003; Koukourakis et al., 2006; Koukourakis et al., 2009). So detection of its enzyme activity in vitro is important for researching on LDH-A. Recently, it has been reported that Lys-5 ...
[摘要] LDH（乳酸脱氢酶）酶使用NAD +作为辅因子催化丙酮酸向乳酸的可逆转化。 尽管乳酸蓄积在作为细胞代谢中的死端产物的肿瘤细胞中的生理学意义目前是一个争论的话题，但长期以来已知许多肿瘤细胞表达高水平的LDH-A（Koukourakis等 et al。，2003; Koukourakis et al。，2006; Koukourakis et al。，2009）。 因此，其体外酶活性的检测对于LDH-A的研究是重要的。 最近，已报道Lys-5乙酰化可降低LDH-A酶活性（Zhao等人，2013）。
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