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Author:
Date:
2014-04-05
[Abstract] α2β1-integrin clustering experiment can be used to trigger internalization of α2β1-integrin. When clustering is performed with sequential administration of primary and fluorescent secondary antibodies, the entry kinetics of integrin can be followed into the cell. The idea is first to allow binding of primary antibodies (recognizing the extracellular epitope) to the α2β1-integrins and then to cluster the α2β1-integrin-bound primary antibodies together by the means of the secondary antibody. Binding is done on ice so that the α2β1-integrins will not internalize before both sets of antibodies ...
[摘要] α2β1整合素聚类实验可用于触发α2β1整联蛋白的内化。 当通过顺序施用原发性和荧光二抗进行聚类时，可以在细胞中进入整联蛋白的进入动力学。 该想法是首先允许一级抗体（识别胞外表位）与α2β1整联蛋白的结合，然后通过二级抗体将α2β1整联蛋白结合的一级抗体聚集在一起。 在冰上进行结合，使得α2β1-整联蛋白在两组抗体结合之前不内化。 聚类已知能够有效地从细胞表面向细胞质触发α2β1整合素内化。 在这个协议中，我们使用抗体诱导聚类的α2β1整合素，以定量内化的α2β1整合素的数量与细胞表面相关的α2β1整合素相比。
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