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Author:
Date:
2014-04-20
[Abstract] Reductive dehalogenation has been found primarily in anaerobic communities and is originally thought to rarely occur in aerobes. A reductive dehalogenase (BhbA) was characterized from an aerobic strain of Comamonas sp. 7D-2, which was isolated from a bromoxynil octanoate-contaminated soil sample collected in Jiangsu, China. BhbA catalyzes the reductive dehalogenation of bromoxynil and its derivative 3,5-dibromo-4-hydroxybenzoate under aerobic conditions. BhbA is membrane-associated and found to have the key features of anaerobic respiratory reductive dehalogenases. This protocol ...
[摘要] 还原脱卤主要在厌氧社区中发现，并且最初被认为很少发生在aerobes中。 还原脱卤素酶（BhbA）的特征在于Comamonas的需氧菌株。 7D-2，其从中国江苏收集的溴苯腈辛酸盐污染的土壤样品中分离。 BhbA在需氧条件下催化溴苯腈及其衍生物3,5-二溴-4-羟基苯甲酸的还原脱卤。 BhbA是膜相关的并且发现具有厌氧呼吸还原脱卤素酶的关键特征。 该方案描述了膜部分中有氧还原脱卤酶（BhbA）的酶分析方法。
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