|
Author:
Date:
2014-07-05
[Abstract] Upon pathogen encounter, naïve CD8+ T cells are primed and undergo massive clonal expansion. A fraction of effector CD8+ T cells remains during the contraction phase and differentiate into memory T cells critical for mounting robust recall responses in response to secondary infection. Low frequency of memory T cells in vivo is a major obstacle to investigate their functional aspects including migration capacity and genetic regulation. Here, we describe detailed protocol for memory T cell differentiation developed by von Andrian’s group to generate large number ...
[摘要] 在病原体遭遇时，初始CD8 + T细胞被引发并经历大量克隆扩增。 效应CD8 + sup T细胞的一部分在收缩阶段期间保持并分化为响应于继发感染而对于安装鲁棒回忆反应至关重要的记忆T细胞。 体内低频记忆T细胞是研究其功能方面（包括迁移能力和遗传调节）的主要障碍。 在这里，我们描述由von Andrian的小组开发的记忆性T细胞分化的详细方案，以在体外产生大量的CD44 - 高 - 抗原特异性记忆T细胞 。
|