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Author:
Date:
2014-08-05
[Abstract] This protocol provides a simple and fast method of quantification for intracellular flavin content, and for flavin secretion by bacteria. Intracellular flavins are extracted from bacterial pellets, and secreted flavins are examined in the cell growth medium. Flavins are separated and measured using HPLC with fluorescence detection, and quantified based on a comparison to standards.
[摘要] 这个协议提供了一种简单和快速的方法定量细胞内黄素含量，和黄素分泌的细菌。 从细菌团块提取细胞内黄素，并在细胞生长培养基中检查分泌的黄素。 分离黄酮并使用具有荧光检测的HPLC测量，并基于与标准品的比较进行定量。
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