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C57BL/6J

C57BL / 6J

公司名称: NCBI
产品编号: 11730008
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Culturing of C57BL/6 Mouse Embryonic Stem (ES) Cell Line
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2011-10-05
[Abstract]  Using ATCC ES C57BL/6 as an example, it is shown here how to culture mouse embryonic stem (ES) cell line. The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008]. The ES cells were shown to populate the germ line of two host blastocyst donors, FVB/NJ (FVB) and the coisogenic strain C57BL/6-Tyrc-2J (c2J). Coat-color chimera production was high using c2J blastocysts while FVB blastocysts produced a low number of chimeras [PubMed: 11730008]. [摘要]  这里以ATCC ES C57BL/6为例介绍如何培养小鼠胚胎干细胞(ES)株系。 编号 #693 ES C57BL/6的干细胞系是从C57BL/6J (B6)小鼠囊胚取得[PubMed: 11730008]。 这些 ES细胞是由两个囊胚供体生殖系移植而来。这两个供体株系分别为FVB/NJ (FVB)和同源株系 C57BL/6-Tyrc-2J (c2J)。使用c2J囊胚株系产生的花色嵌合产物多于使用FVB囊胚株系[PubMed: 11730008]。

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