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Xylene

二甲苯

公司名称: Wako Pure Chemical Industries
产品编号: 244-00086
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Detection of Poly (A) RNA in Mesophyll Cells of Nicotiana benthamiana Using in situ Hybridization
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2015-09-05
[Abstract]  Export of transcribed mRNAs from nucleoplasm to cytosol is an essential process for the translation of genes into proteins. This process is tightly regulated by nuclear pores, composed of about 30 nucleoporin proteins (Nups). Whether or not the mRNAs are able to be appropriately exported to cytoplasm is of an importance for understanding the role of Nups. Here, we describe a practical protocol to detect the intracellular localization of mRNAs in mesophyll cells of Nicotiana benthamiana (N. benthamiana). This protocol is based on poly (A) in situ hybridization method ... [摘要]  将转录的mRNA从核质到细胞质的输出是将基因翻译成蛋白质的必要过程。 这个过程受到由约30个核蛋白(Nups)组成的核孔紧密调节。 mRNA是否能够适当地输出到细胞质对于理解Nups的作用是重要的。 在这里,我们描述一种实用的协议,以检测本地烟草的本底细胞中的mRNA的细胞内定位(<本宁顿烟草)。 该方案基于使用与alexa fluor-488缀合的寡聚d(t)探针的poly(a)原位杂交方法。 该方案基于使用与alexa="" fluor-488缀合的寡聚d(t)探针的poly(a)原位杂交方法。="">

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