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Dialysis tubing, high retention seamless cellulose tubing

透析管,高保留无缝纤维素管

公司名称: Sigma-Aldrich
产品编号: D0530
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Preparation of Arabinogalactan Glycoproteins from Plant Tissue
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2013-10-05
[Abstract]  This supplements an earlier protocol (Popper, 2011) for the extraction and assay of cell surface arabinogalactan proteins (AGPs). These highly glycosylated glycoproteins (~95% carbohydrate) contain numerous glycomodules with paired glucuronic acid residues that bind Ca2+ in a pH dependent manner (Lamport and Varnai, 2013). Classical AGPs comprise the bulk of cell surface glycoproteins and are thus integral components of a Ca2+ oscillator involved in a signalling pathway where calcium is a “universal signalling currency” analogous to ATP as the universal energy currency. ... [摘要]  这补充了用于细胞表面阿拉伯半乳聚糖蛋白(AGP)的提取和测定的早期方案(Popper,2011)。这些高度糖基化的糖蛋白(〜95%碳水化合物)含有许多具有以pH依赖性方式结合Ca 2+ 2+的成对葡萄糖醛酸残基的糖模块(Lamport和Varnai,2013)。经典AGP包含大量细胞表面糖蛋白,因此是参与信号传导途径的Ca 2+ 2+振荡器的整体组分,其中钙是类似于作为通用能量货币的ATP的"通用信号传导货币"。因此,这些外周糖蛋白的中心作用是足以进行进一步研究的理由。然而,由于广泛的糖基化及其明显的微不均一性,通常假定排除简单的还原剂方法而出现问题。在这里,我描述了基于其与β-D-葡糖基或半乳糖基的特异性相互作用的经典AGP的简单部分纯化Yariv试剂,一种合成的重氮染料,其在中性pH的盐溶液中沉淀AGP作为不溶性复合物。 ...

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