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Author:
Date:
2015-12-20
[Abstract] The long-distance translocation of metabolites and mineral elements is crucial for plant growth and reproduction. In most cases, source-to-sink translocation of metabolites and minerals requires their passage through the apoplast, irrespective whether they are transported via the xylem or the phloem. This apoplast-mediated pathway is of particular importance during plant senescence, when photoassimilates as well as organic, inorganic or chelated forms of nutrients are translocated from leaves to fruits or seeds. Recent genetic and physiological studies revealed the involvement of numerous ...
[摘要] 代谢物和矿物元素的长距离移位对于植物生长和繁殖是至关重要的。在大多数情况下，代谢物和矿物质的源到汇转移需要它们通过质外体，不管它们是通过木质部或韧皮部转运。这种质外体介导的途径在植物衰老期间特别重要，当光同化物以及有机，无机或螯合形式的营养物从叶子转移到水果或种子时。最近的遗传和生理研究揭示了许多膜转运蛋白介导氨基酸，糖，尿素或矿物元素的韧皮部载荷的参与。为了评估单个转运蛋白对木质部卸载或韧皮部负荷的贡献，外质体液和韧皮部液体的收集是必需的。在这里，我们描述了从拟南芥叶中提取质外体液和叶柄渗出物的方法，后者代表韧皮部汁液的真实组成的近似。
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