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Author:
Date:
2016-01-05
[Abstract] Low-molecular-weight (LMW) thiols are a class of highly reactive compounds due to their thiol moiety. They play important roles in the maintenance of cellular redox homeostasis, detoxification, and development. Monobromobimane (mBBr) is weakly fluorescent but selectively reacts with thiols to yield highly fluorescent thioethers (mBSR) products, which is especially useful for the quantification of LMW thiols. The stable mBSR products can be separated by high-performance liquid chromatography (HPLC) equipped with a fluorescent detector. The main cellular LMW thiols are L-cysteine, ...
[摘要] 低分子量（LMW）硫醇是一类高反应性化合物，由于它们的硫醇部分。 它们在维持细胞氧化还原稳态，解毒和发育中起重要作用。 单溴溴烷（mBBr）是弱荧光的，但选择性地与硫醇反应产生高荧光硫醚（mBSR）产物，其对于定量LMW硫醇特别有用。 稳定的mBSR产物可以通过配备有荧光检测器的高效液相色谱（HPLC）分离。 主要的细胞LMW硫醇是L-半胱氨酸，γ-谷氨酰半胱氨酸和谷胱甘肽（GSH）。 以下方案描述了从所报道的拟南芥组织中提取和定量L-半胱氨酸，γ-谷氨酰半胱氨酸和谷胱甘肽（Xiang和Oliver，1998; Zhao等人， 2014; Wang ，，2015）。 如果HPLC系统或C18柱不同，可能需要进行修改。
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