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Adenosyl-L-Methionine, S-[methyl-3H]-, Specific Activity: 55-85Ci (2.03-3.15TBq)/mMole, 1mCi (37MBq)

腺苷酰-L-甲硫氨酸,S- [甲基 - 3 H] - ,比活性:55-85Ci(2.03-3.15TBq)/ mMole,1mCi(37MBq)

公司名称: PerkinElmer
产品编号: NET155H001MC
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Biochemical Assays for MTase Activity
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2014-01-20
[Abstract]  Methyltransferase (MTase) transfers a methyl group (-CH3) from the donor S-adenosyl-L-methionine (AdoMet or SAM) to biologically active molecules such as hormones, neurotransmitters, lipids, proteins and nucleic acids. The addition of a methyl group causes a change in the physicochemical properties of the molecules. The mRNA cap structure is essential for cell and virus. Guanine-N7-methyltransferase (N7-MTase) methylates the GpppN cap at the N7 position of guanine, resulting in cap-0 structure (m7GpppN), and Ribose 2'-O-MTase further methylates the first nucleotide of higher eukaryotic ... [摘要]  甲基转移酶(MTase)将甲基(-CH 3)从供体S-腺苷-L-甲硫氨酸(AdoMet或SAM)转移到生物活性分子例如激素,神经递质,脂质,蛋白质和核酸。 加入甲基引起分子的物理化学性质的改变。 mRNA帽结构对于细胞和病毒是必需的。 鸟嘌呤-N7-甲基转移酶(N7-MTase)甲基化在鸟嘌呤的N7位置的GpppN帽,导致cap-0结构(m7GpppN),核糖2'-O-MTase进一步甲基化高等真核细胞和病毒的第一个核苷酸 mRNA在核糖2'-OH位置形成cap-1(m7GpppNm)结构。 在这里,我们描述一种生物化学测定检测mRNA上限MTases的活动。

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