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Perfect DNATM 100 bp Ladder

DNA TM 100 bp Ladder

公司名称: EMD Millipore
产品编号: 70539
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Generation of Non-typeable Haemophilus influenzae Directed Gene Deletion Mutants
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2014-03-05
[Abstract]  Directed deletion mutants in non-typeable Haemophilus influenzae can be made by allelic exchange of the target gene with an artificial DNA construct in which an antibiotic resistance cassette is placed between two ~1,000 bp DNA sequences that are identical to the 5' and 3' flanking regions of the target gene. The artificial DNA construct that is required for this mutagenesis is synthesized by the so-called Megaprimer PCR method (Figure 1).
[摘要]  在非典型流感嗜血杆菌中的定向缺失突变体可以通过靶基因与人工DNA构建体的等位基因交换来制备,其中抗生素抗性盒置于两个〜1000bp的DNA序列之间, 靶基因的5'和3'侧翼区。 该诱变所需的人工DNA构建体通过所谓的Megaprimer PCR方法合成(图1)。

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