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Next-generation protease inhibitor cocktail tablets

Next-generation protease inhibitor cocktail tablets

公司名称: Roche Diagnostics
产品编号: 05 892 970 001
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Catalase Activity Assay in Candida glabrata
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2014-03-20
[Abstract]  Commensal and pathogenic fungi are exposed to hydrogen peroxide (H2O2) produced by macrophages of the host. Pathogenic fungi counteract the harmful effects of H2O2 with the enzyme catalase (EC 1.11.1.6), which decomposes two molecules of H2O2 to two molecules of H2O and O2. Contribution of antioxidant systems on fungal virulence is actively studied. Measurement of catalase activity can contribute to the elucidation of the factors that influence the regulation of this pivotal enzyme. Here we describe a ... [摘要]  共生和致病真菌暴露于由宿主的巨噬细胞产生的过氧化氢(H 2 O 2 O 2)。 致病真菌抵消了H 2 O 2对于过氧化氢酶(EC 1.11.1.6)的有害影响,所述过氧化氢酶分解两个分子的H 2 O 2 - O 2至两个H 2 O和O 2分子。 积极研究抗氧化系统对真菌毒力的贡献。 过氧化氢酶活性的测量可有助于阐明影响这种关键酶的调节的因素。 在这里我们描述一个简单的分光光度法,其中过氧化氢酶的活性在总酵母提取物中测量。 通过酵母提取物的H 2 O 2 O 2分解后,在240nm处的吸光度降低。 吸光度随时间的差异(ΔA240)被推断为过氧化氢酶活性的量度。

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