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Ruthenium Red

钌红

公司名称: Sigma-Aldrich
产品编号: R2751
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Seed Coat Ruthenium Red Staining Assay
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Date:
2014-04-05
[Abstract]  The goal of this protocol is to assay for defects in synthesis/secretion/release of seed coat mucilage by ruthenium red staining of mature whole seeds.

The mucilage secretory cells of the Arabidopsis seed coat synthesize and secrete a large quantity of primarily pectinaceous mucilage to a ring-shaped apical domain during their differentiation. This makes them an excellent model system to identify genes involved in both cell wall synthesis and secretion (et al., 2000). When wild-type seeds are incubated in ruthenium red stain, hydrated mucilage is extruded from ...
[摘要]  该协议的目的是通过成熟全种子的钌红染色来测定种皮粘液合成/分泌/释放中的缺陷。拟南芥种皮的粘液分泌细胞合成 并在其分化期间将大量的主要果胶粘液分泌到环状顶端结构域。 这使得它们是鉴定参与细胞壁合成和分泌的基因的极好的模型系统(等人,2000)。 当野生型种子在钌红染色中孵育时,水合粘液从表皮细胞中挤出,并且在种子周围观察到红色染色的粘液的"晕"(Western等人,2000)。 减少粘液染色可能由细胞壁生物合成,分泌缺陷或水合作用时释放受损而导致。

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