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ORCA-R2 Digital CCD camera

ORCA-R2数字CCD摄像机

公司名称: Hamamatsu Photonics
产品编号: C10600-10B
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Time-lapse Observation of Chromosomes, Cytoskeletons and Cell Organelles during Male Meiotic Divisions in Drosophila
Author:
Date:
2017-04-20
[Abstract]  In this protocol, we provide an experimental procedure that perform time-lapse observation of intra-cellular structures such as chromosomes, cytoskeletons and cell organelles during meiotic cell divisions in Drosophila males. As primary spermatocyte is the largest dividing diploid cell in Drosophila, which is equivalent in size to mammalian cultured cells, one can observe dynamics of cellular components during division of the model cells more precisely. Using this protocol, we have showed that a microtubule-associated protein plays an essential role in microtubule dynamics ... [摘要]  在这个协议中,我们提供了一个实验程序,在果蝇男性的减数分裂细胞分裂期间,进行细胞内结构如染色体,细胞骨架和细胞器的延时观察。由于主要精母细胞是果蝇中最大的分裂二倍体细胞,其大小与哺乳动物培养细胞相当,可以更准确地观察模型细胞分裂期间细胞组分的动力学。使用该方案,我们已经表明,微管相关蛋白在微管动力学中起重要作用,并通过微管和肌动球蛋白丝之间的相互作用开始裂解沟槽。我们还报道说,需要核膜组分来形成和/或维持在果蝇细胞中细胞分裂所必需的主轴包络。

在果蝇中,也可以在标准培养条件下良好培养的良好培养细胞系。然而,它们的单元尺寸,特别是细胞质体积,比哺乳动物细胞的小得多。这在细胞分裂过程中损害了细胞成分的检查。精母细胞,在另一方面,实现第一次减数分裂开始之前不同的细胞生长。主要精母细胞是出现在果蝇发育中的增殖细胞中最大的二倍体细胞。因此,可以使用光学显微镜容易地细分观察分裂细胞中的细胞结构。在果蝇黑腹果蝇中,提供了先进和复杂的遗传技术(Ashburner等人,2004)。染色体分离和细胞分裂中的减数分裂缺陷出现在完成2< 减数分裂后精子细胞的细胞组织中。通过观察这种早期精子细胞,人们可以很容易地发现甚至微小的减数分裂异常(2012); 2012年; ...

Rice Meiotic Chromosome Spread Preparation of Pollen Mother Cells
Author:
Date:
2014-07-20
[Abstract]  In this protocol, we describe a simple and efficient method for meiotic chromosome spread preparation in rice pollen mother cells. Meiotic chromosome preparation by spreading itself is an important technique for plant cytogenetics (Higgins et al., 2004; Chelysheva et al., 2012; Wang et al., 2009); furthermore, it is a crucial step for applying other cytogenetic methods including Fluorescence in situ hybridization (FISH) and immunostaining.
[摘要]  在这个协议,我们描述一种简单和有效的方法,在水稻花粉母细胞减数分裂染色体传播准备。 通过铺展本身的减数分裂染色体制备是植物细胞遗传学的重要技术(Higgins等人,2004; Chelysheva等人,2012; Wang等人,/em>,2009); 此外,它是应用其他细胞遗传学方法包括荧光原位杂交(FISH)和免疫染色的关键步骤。

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