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Low Diffusion PE Vial, 20 mL, Anti-Static

低扩散PE瓶

公司名称: PerkinElmer
产品编号: 6000477
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Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
Author:
Date:
2016-11-20
[Abstract]  The choline-containing phospholipid, phosphatidylcholine (PtdCho) is the most common mammalian phospholipid found in cell membrane (Ide et al., 2013). It is also a component of intracellular signalling pathways (Cui and Houweling, 2002). Herein is described a measure of the rate of accumulation of choline by lipid soluble PtdCho and lyso-Ptdcho which can further be discriminated by chromatographic analysis (Smith and Phyu, 2016). Determination of the accumulation of [3H-methyl]-choline into water-soluble components is also described. The procedure could be used to measure ... [摘要]  含胆碱的磷脂,磷脂酰胆碱(PtdCho)是在细胞膜中发现的最常见的哺乳动物磷脂(Ide等人,2013)。它也是细胞内信号传导途径的组成部分(Cui和Houweling,2002)。本文描述了脂质可溶性PtdCho和溶血磷脂酰胆碱积累胆碱的速率的量度,其可通过色谱分析进一步区分(Smith和Phyu,2016)。还描述了[3 H] - 甲基] - 胆碱在水溶性组分中的积累的测定。该程序可用于测量药物和其它因素对胆碱掺入磷脂的影响。在将细胞暴露于测试条件(例如,药物)后,将组织培养瓶中的贴壁细胞与放射性标记的[3 H] - 甲基] - 胆碱在培养基中温育15分钟(脉冲)。然后快速除去[3 H] - 甲基] - 胆碱,并在非放射性培养基(Chase)存在下继续孵育。然后通过细胞分级和测量脂质和非脂质细胞组分中的放射性来测定[3 H] - 甲基的细胞分布。

[背景] 磷脂代谢在细胞膜形成中是必需的(Ide等人,2013)和细胞信号传导(Cui和Houweling, 2002)。包含胆碱的代谢物的形成和胆碱在脂质中的积累是细胞增殖过程中的关键过程。磷脂代谢紊乱与癌症和其他疾病有关(Gibellini和Smith,2010)。这些过程的测量对于理解医学成像模态至关重要,所述医学成像模式使用[11 C] - ...

In vitro DNA Polymerization Activity Assay Using Cell-free Extracts
Author:
Date:
2014-08-20
[Abstract]  This protocol has been designed to measure the in-vitro DNA polymerization activity in crude cell extracts of the Antarctic bacterium Pseudomonas syrinagae Lz4W. This bacterium can grow at 4 °C with optimum growth rate at 22 °C. The slow growth rate of the bacterium observed at low temperature (4 °C) compared to higher temperature (22 °C) can be attributed to the reduced rate of DNA replication at low temperature. Here we describe a protocol which we have used to quantify the in vitro DNA polymerization of cell extracts at two different temperatures. [摘要]  该方案已经设计用于测量南极细菌假单胞菌syrinagae Lz4W的粗细胞提取物中的体外 DNA聚合活性。 该细菌可以在4℃下生长,在22℃以最佳生长速率生长。 在低温(4℃)下观察到的细菌与较高温度(22℃)相比的缓慢生长速率可以归因于在低温下DNA复制的速率降低。 在这里我们描述了一个协议,我们已经用来量化的体外细胞提取物在两个不同的温度下的DNA聚合。

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