| Cell Adhesion Assay
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Author:
Date:
2012-03-05
[Abstract] Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a range of biological events such as three-dimensional re-organization of the cytoskeleton, biochemical reactions in the cell, and changes in molecules on the surface of the cell. Cancer cells, especially the highly metastatic types, are believed to have enhanced adhesion ability that often facilitates the migration of the cells to a new site to ...
[摘要] 修改的细胞吸附试验方法是在对细胞与胞外物质相互作用的研究下得出的。
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| Scratch Wound Healing Assay
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Author:
Date:
2012-03-05
[Abstract] The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation. In a typical scratch wound healing assay, a “wound gap” in a cell monolayer is created by scratching, and the “healing” of this gap by cell migration and growth towards the center of the gap is monitored and often quantitated. Factors that alter the motility and/or growth of the cells can lead to increased or decreased rate of “healing” of the gap (Lampugnani, 1999). ...
[摘要] 细胞伤口愈合实验被研究者广泛应用和改进，用来研究各种实验条件比如基因敲除和化疗在细胞迁移和增殖中的作用。该实验操作简单，费用廉价，实验条件容易根据不同的目的改进。该方法的基本原理是，在单层培养细胞间制作划痕以产生愈伤区域，然后监控该伤口周围细胞的向划痕迁移的现象，即伤口愈合。改变细胞迁移和/或生长的因素可以增加或降低伤口愈合率。该方法可以定量，适用于自动化系统高通量筛选。
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| Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
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Author:
Date:
2012-02-20
[Abstract] Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. The assay is also useful in screens for compounds that act as chemoattractants or inhibitors of chemotaxis for cells. The assay employs a permeable layer of support, usually a tissue-culture-treated microporous membrane, which is positioned between two compartments that mimic two different sets of microenvironments for cell survival/growth. Cells on one side of the membrane, when sensing chemoattractants placed on the other side of the compartment that ...
[摘要] 小室迁移实验已被广泛用于研究不同类型包括转移性肿瘤细胞的细胞活力。该实验对检验趋化因子或细胞趋化抑制剂的化合物是有用的。检测依赖于透水层的支持，通常这是组织培养处理的微孔滤膜，它位于两个小室之间，模仿两个不同的微环境对细胞生存/生长。细胞在膜的一侧，从小室的另一侧感应的趋化因子，可以通过微孔膜迁移。被移植的细胞可以用简单的固定/染色的计数方法量化。人类乳腺上皮腺癌MD - 231细胞生长比较快，是转移性的。 以下就以MB - 231细胞细胞株为例描述细胞迁移实验的基本程序。
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