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Anti-wide spectrum Cytokeratin antibody

抗宽光谱细胞角蛋白抗体

公司名称: Abcam
产品编号: ab9377
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Three Dimensional Spheroid Co-culture Invasion Assay
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2015-01-05
[Abstract]  The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells (in our case breast cancer cell lines) (Verghese et al., 2013). Typical two dimensional invasion assays do necessarily account for the presence of extracellular matrix that is present around the stromal and tumour cells in vivo and therefore cellular behaviour within these cultures may be non-physiological. This spheroid assay ... [摘要]  开发该测定以研究不同类型(在我们的情况下乳腺癌相关的成纤维细胞稳定操纵以修饰目的基因的表达)的基质细胞对上皮癌细胞(在我们的乳腺癌细胞系)的侵袭能力的影响( Verghese ,,2013)。典型的二维侵袭测定确实需要考虑在体内基质和肿瘤细胞周围存在的细胞外基质的存在,因此这些培养物中的细胞行为可能是非生理的。开发这种球状体测定以尝试更密切地复制存在于实际肿瘤中的乳腺癌基质和肿瘤细胞周围的环境(Verghese等人,2013)。包括由胶原IV和胶原I组成的细胞外基质,并且成纤维细胞和上皮细胞被给予发展"生理"相互作用的机会(Verghese等人,2013; Hooper等人, ,2006)。该方法由Nowicki等人(2008)开发,并且在Verghese等人(2013)中使用它来公开数据。

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