Author:
Date:
2015-03-05
[Abstract] Zebrafish have become a popular animal model for behavioral neuroscience (Gerlai, 2014). Recent studies have demonstrated that brief experimental handling prior to euthanizing animals can subsequently alter biological measures quantified post-mortem (e.g. cortisol levels) (Ramsay et al., 2009; Tran et al., 2014). Here we provide a detailed protocol for a simple 30-sec net stressor task for adult zebrafish that increases whole-body cortisol levels without altering the levels of whole-brain dopamine, 3, 4-dihydroxyphenylacetic acid, serotonin, and ...
[摘要] 斑马鱼已成为行为神经科学的流行动物模型(Gerlai,2014)。 最近的研究已经证明,在安乐死动物之前的短暂的实验操作可以随后改变生后量化的生物学测量(例如皮质醇水平)(Ramsay等人,2009; em> et al。,2014)。 在这里我们提供一个简单的30秒净应激子任务的成年斑马鱼,增加全身皮质醇水平,而不改变全脑多巴胺,3,4-二羟基苯乙酸,血清素和5-羟基吲哚乙酸的水平的简单协议 Tran et al。,2014)。
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