Nonidet-P40 substitute (Igepal CA-630, T-DET O-series, ethoxylated octylphenol, T-DET O-9)

Nonidet-P40替代物（Igepal CA-630，T-DET O系列，乙氧基化辛基苯酚，T-DET O-9）

公司名称: United States Biological

产品编号: N3500

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Analysis of Phagosomal Antigen Degradation by Flow Organellocytometry

Eik Hoffmann, Anne-Marie Pauwels, Andrés Alloatti, Fiorella Kotsias and Sebastian Amigorena

Published online: 2016-11-20

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In vitro Ag Cross-presentation and in vivo Ag Cross-presentation by Dendritic Cells in the Mouse

Author:

Mallika Ghosh and Linda H Shapiro,

Date:

2012-12-20

[Abstract] Antigen cross presentation is important for effective immune responses to tumors and viral infections. Dendritic cells are professional antigen presenting cells and are unique in their ability to cross-present exogenous antigens on MHC class I molecules and activate antigen specific cytotoxic T cells. This protocol describes antigen cross presentation by dendritic cells (DCs) (bone marrow derived DCs and splenic DCs) in an in vitro and in an in vivo assay system using soluble ovalbumin protein. [摘要] 抗原交叉呈递对于对肿瘤和病毒感染的有效免疫应答是重要的。树突状细胞是专业抗原呈递细胞，并且在其将MHC I类分子上的外源抗原交叉呈递并激活抗原特异性细胞毒性T细胞的能力方面是独特的。该方案描述了在体外和在体内使用可溶性卵白蛋白蛋白质的体系中通过树突细胞（DC）（骨髓来源的DC和脾DC）的抗原交叉呈递。

Restriction Enzyme Accessibility Protocol in Mammalian Cells

Author:

Hogune Im,

Date:

2011-10-20

[Abstract] This protocol describes a method to indirectly assess chromatin structure by using restriction enzyme in mammalian cells, modified from Current Protocols. [摘要] 本实验方法介绍了一种通过使用哺乳动物细胞中限制性内切酶间接评估染色体结构的方法，修改自Current Protocols. 注意：不要在第1-18步中间停下。

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Nonidet-P40 substitute (Igepal CA-630, T-DET O-series, ethoxylated octylphenol, T-DET O-9)

Nonidet-P40替代物（Igepal CA-630，T-DET O系列，乙氧基化辛基苯酚，T-DET O-9）

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