| Preparation of Single Cell Suspensions from Mouse Aorta
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Author:
Date:
2016-06-05
[Abstract] Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery tertiary lymphoid organs in the adventitia (Grabner et al., 2009; Hu et al., 2015). Various and diverse types of immune cells have been identified in the aorta adventitia vs atherosclerotic plaques (Elewa et al., 2016; Galkina et al., 2006; Lotzer et al., 2010; ...
[摘要] 动脉粥样硬化是动脉壁的慢性炎性疾病，其特征在于脂质沉积，斑块形成和免疫细胞浸润。先天性和适应性免疫细胞在疾病发展期间浸润动脉。此外，晚期疾病导致外膜中动脉三级淋巴器官的形成（Grabner等人，2009; Hu等人，2015）。已经在主动脉外膜vs动脉粥样硬化斑块中鉴定了各种不同类型的免疫细胞（Elewa等人，2016; Galkina等人，2006; Lotzer等人， 2010; Mohanta等人，2016; Mohanta等人，2014; Moos等人，2010; Mohanta等人，2010; 2005; Srikakulapu et al。，2016; Zhao et al。，2004）。根据动物的年龄，用于获得单细胞悬浮液的方案和小鼠的饮食条件，存在关于主动脉中免疫细胞的数量和亚型的矛盾报告（Campbell等， 2012; Clement等人，2015; Galkina等人，2006; Kyaw等人，2012）。使用不同的方案，主动脉中免疫细胞的数目差异多达十倍（Butcher等，2012; Galkina等，2006; Gjurich等，2015; Grabner等人，2009; ...
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| Isolation of Murine Adipose Tissue-derived Mesenchymal Stromal Cells (mASCs) and the Analysis of Their Proliferation in vitro
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Author:
Date:
2015-11-05
[Abstract] Mesenchymal stromal cells (MSCs) are non-hematopoietic, perivascular cells which support hematopoiesis and are thought to participate in tissue repair in vivo. MSCs can be isolated from various tissues and organs and are defined in vitro as plastic adherent cells expressing CD73, CD90, CD105 (human MSCs) or CD29, CD44, sca-1 (murine MSCs) which can differentiate into osteoblasts, adipocytes, chondroblasts and myocytes. MSCs possess potent immunomodulatory and trophic capacities in vitro and in vivo and have thus emerged as a promising treatment of ...
[摘要] 间充质基质细胞（MSC）是非造血的血管周围细胞，其支持造血并且被认为在体内参与组织修复。 MSC可以从各种组织和器官中分离并在体外定义为表达CD73，CD90，CD105（人MSC）或CD29，CD44，sca-1（鼠MSC）的塑性贴壁细胞，其可以分化成骨细胞，脂肪细胞，软骨细胞和肌细胞。 MSC在体外具有强的免疫调节和营养能力，并且在体内具有强的免疫调节能力，因此已经成为炎症/自身免疫疾病的有希望的治疗。 MSC对人类疾病的使用依赖于大量细胞的注射，并且许多努力已经集中在获得具有高增殖能力的MSC。因此，建立简单和准确的测定MSC增殖的协议对于基础研究和应用研究都是重要的。目前的方案提供了关于如何使用xCELLigence系统和CellTiter-Blue试剂（Carrillo-Galvez等人，）分离和测量源自腹股沟和/或腹内脂肪组织（mASCs）的鼠MSC的增殖的细节>，2015; Anderson ，，2013）。下面描述的方案也可以容易地翻译成人MSC。
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| In vivo Lineage-tracing Studies in a Cancer Stem Cell Population in Neuroblastoma
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Author:
Date:
2014-04-20
[Abstract] Tumors are comprised of heterogeneous subpopulations that may exhibit differing capacity for differentiation, self-renewal, and tumorigenicity. In vivo lineage-tracing studies are a powerful tool for defining the role of tumor subpopulations in tumor growth and as targets for therapeutic agents. This protocol describes using a neuroblastoma cancer cell line transduced with two different fluorescent proteins (GFP and tdTomato) to track the specific contributions of cells expressing the GCSF receptor (CD114+) or not (CD114-) on tumor growth in vivo.
[摘要] 肿瘤由异质亚群组成，其可以表现出不同的分化，自我更新和致瘤性能力。 体内谱系追踪研究是定义肿瘤亚群在肿瘤生长中的作用和作为治疗剂靶标的有力工具。 该方案描述了使用用两种不同的荧光蛋白（GFP和tdTomato）转导的神经母细胞瘤癌细胞系来追踪表达GCSF受体（CD114 + sup）+或CD114（sup）+的细胞的特异性作用 ）对肿瘤生长的作用。
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