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LIVE/DEAD® Fixable Blue Dead Cell Stain Kit, for UV excitation

可固定的蓝色死细胞污点成套工具

公司名称: Thermo Fisher Scientific
产品编号: L23105
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Quantitative Measurements of HIV-1 and Dextran Capture by Human Monocyte-derived Dendritic Cells (MDDCs)
Author:
Date:
2016-11-20
[Abstract]  The aim of this protocol is to describe how to measure and quantify the amount of HIV-1 particles and dextran molecules internalized in human monocyte derived dendritic cells (MDDCs), using three different techniques: flow cytometry, quantitative PCR and confocal microscopy. [摘要]  本协议的目的是描述如何使用三种不同的技术:流式细胞术,定量PCR和共聚焦显微镜来测量和量化人类单核细胞衍生树突细胞(MDDCs)中内在的HIV-1颗粒和葡聚糖分子的量。

[背景] 开发此协议是为了评估在人单核细胞衍生的树突细胞中肌动蛋白成核破坏时HIV-1内化的变化。在shRNA筛选后,识别对于HIV-1从树突状细胞转移到T细胞重要的基因,我们观察到肌动蛋白成核的中断导致从富含肌动蛋白的树突到水泡的转变,由于过量的肌动球蛋白收缩。结果,观察到HIV-1转移的减少和由于水泡收缩驱动的巨噬细胞增多引起的HIV-1内化的增加。我们的结论是,肌动蛋白成核和稳定的效应器是维持艾滋病毒1对肌动蛋白丰富的树突和限制其内吞作用,有效转移到T淋巴细胞的关键(Menager和Littman,2016)。

Preparation of Single Cell Suspensions from Mouse Aorta
Author:
Date:
2016-06-05
[Abstract]  Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery tertiary lymphoid organs in the adventitia (Grabner et al., 2009; Hu et al., 2015). Various and diverse types of immune cells have been identified in the aorta adventitia vs atherosclerotic plaques (Elewa et al., 2016; Galkina et al., 2006; Lotzer et al., 2010; ... [摘要]  动脉粥样硬化是动脉壁的慢性炎性疾病,其特征在于脂质沉积,斑块形成和免疫细胞浸润。先天性和适应性免疫细胞在疾病发展期间浸润动脉。此外,晚期疾病导致外膜中动脉三级淋巴器官的形成(Grabner等人,2009; Hu等人,2015)。已经在主动脉外膜vs动脉粥样硬化斑块中鉴定了各种不同类型的免疫细胞(Elewa等人,2016; Galkina等人,2006; Lotzer等人, 2010; Mohanta等人,2016; Mohanta等人,2014; Moos等人,2010; Mohanta等人,2010; 2005; Srikakulapu et al。,2016; Zhao et al。,2004)。根据动物的年龄,用于获得单细胞悬浮液的方案和小鼠的饮食条件,存在关于主动脉中免疫细胞的数量和亚型的矛盾报告(Campbell等, 2012; Clement等人,2015; Galkina等人,2006; Kyaw等人,2012)。使用不同的方案,主动脉中免疫细胞的数目差异多达十倍(Butcher等,2012; Galkina等,2006; Gjurich等,2015; Grabner等人,2009; ...

Isolation and Purification of Murine Microglial Cells for Flow Cytometry
Author:
Date:
2016-01-05
[Abstract]  The detailed protocol is used to isolate different cell types from murine brain as glial cells, including microglia, using an enzymatic digestion that minimizes cellular mortality. A Percoll gradient (30% to 80%) separation allows a maximal recovery of isolated murine microglial cells prior to flow cytometry analysis. [摘要]  详细的协议是用于分离不同的细胞类型从鼠脑作为神经胶质细胞,包括小胶质细胞,使用最小化细胞死亡率的酶消化。 Percoll梯度(30%至80%)分离允许在流式细胞术分析之前分离的小鼠胶质细胞的最大恢复。

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