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Histone from calf thymus

来自小牛胸腺的组蛋白

公司名称: Sigma-Aldrich
产品编号: H9250
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Immunoblot Analysis of Histone H4 Acetylation and Histone H2A Phosphorylation in Candida albicans
Author:
Date:
2013-10-20
[Abstract]  Posttranslational modifications of histones are required for different processes including transcription, replication and DNA damage repair. This protocol describes the preparation of a whole-cell extracts for the fungal pathogen Candida albicans. Furthermore, the extract is used to detect lysine acetylation of histone H4 as well as serine 129 phosphorylation of histone H2A by immunoblot analysis.
[摘要]  包括转录,复制和DNA损伤修复的不同过程需要组蛋白的翻译后修饰。 该方案描述了真菌病原体白色念珠菌的全细胞提取物的制备。 此外,提取物用于通过免疫印迹分析检测组蛋白H4的赖氨酸乙酰化以及组蛋白H2A的丝氨酸129磷酸化。

Histone Methyltransferase Assay in vitro
Author:
Date:
2012-12-20
[Abstract]  Histone methylation is an important epigenetic modification that plays important roles in plant development and growth. Histone methytransferases are the enzymes to establish histone methylation and here we describe a simple and effective protocol for detecting methyltransferase activity and specificity in vitro. [摘要]  该实验方案的中文版正在准备中...

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