| Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-cardiolipin Antibodies
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Author:
Date:
2012-06-20
[Abstract] The circulating anticardiolipin antibody is a hallmark of antiphospholipid syndrome. It also appears in a number of autoimmune mouse models and is indicative of the break of tolerance against self antigens. This protocol describes a reliable method to determine the relative serum titer of anticardiolipin in autoimmune mouse models.
[摘要] 抗心磷脂抗体循环是抗磷脂综合症的标志。它也表现在一些自身免疫鼠模型中并且显示自身抗原耐受的崩溃。这个规程描述了一个可靠的在自身免疫鼠模型中检测抗心磷脂相关血清滴度的方法。
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| Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
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Author:
Date:
2012-06-20
[Abstract] Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol describes a reliable method to determine the relative serum titers of anti-histone antibodies in these mice.
[摘要] 自身免疫疾病是以自身抗原耐受的崩溃为特征。在大多数系统性红斑狼疮(SLE)病人和一些狼疮易感鼠中能找到抗组蛋白抗体，这个规程描述了一个可靠的在这些鼠中检测相关的抗组蛋白抗体血清滴度的方法。
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| Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-dsDNA Antibodies
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Author:
Date:
2011-02-20
[Abstract] ELISA is an easy and relatively sensitive way to measure protein concentration. This protocol describes how to measure serum levels of murine anti-dsDNA antibodies. Circulating anti-dsDNA antibody is a hallmark of SLE both in human patients and in many SLE mouse models. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.
[摘要] 酶联免疫吸附测定是一种简单且相对灵敏的检测蛋白浓度的方法。这个实验步骤描述了如何用酶联免疫吸附测定的方法来检测鼠科动物体内双链DNA抗血清的水平。无论是在系统性红斑狼疮患者体内还是患有此病的模式小鼠体内，双链DNA抗体都会在体内循环，这是系统性红斑狼疮病的一个显著特征。它是由Feinstein医学研究院Anne Davidson博士实验室修改完善的。
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