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siGENOME Non-Targeting siRNA Control Pools

siGENOME非靶向siRNA控制池

公司名称: Cytiva
产品编号: D-001206-13
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Dharmacon siRNA Transfection of HeLa Cells
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2012-02-20
[Abstract]  Small Interfering RNA (siRNA) is a class of double-stranded RNAs of 20-25 nucleotides that play important roles in many biological processes (Hamilton and Baulcombe, 1999). siRNAs act by “neutralizing” the mRNA of the target protein, facilitating degradation of the mRNA and hence altering the biological effect of the protein (reviewed in Hannon and Rossi, 2004). siRNAs may also change the intracellular levels of regulatory RNAs. Use of siRNAs for manipulating the expression of genes of interest in biological research is commonly referred to as RNA interference or knockdown technique (Elbashir et ... [摘要]  小干扰RNA (siRNA)是一类20~25nt大小的双链RNA,在生物体内起着重要的作用 [1]. 人工合成的siRNAs 已经成为一个有效的工具广泛的应用于调控基因转录和翻译,它可以作用于感兴趣的靶向蛋白使其mRNA失效(“敲除”),这一过程通常被称为RNA干扰[2].RNA干扰(敲除)是一个使靶向RNA降解的复杂过程[3 & refs therein]. 对同一靶向RNA,通过不同的公司可能设计的干扰小RNA不同,这里介绍一种用Dharmacon (Thermo)的 siGENOME SMARTpool试剂介导的siRNA转染的实验方法 。

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